Psicheck-2 promega
WebFeb 21, 2024 · In the psiCHECK-2 vector, Renilla is expressed under SV40_promotor/enchancer and is a test luciferase, whereas Firefly is expressed under HSV-TK promoter and is an endogenous control. Therefore,... WebAnalyze Sequence: psiCHECK-2. Sign Up for Our Newsletter. Receive the latest news, hot plasmids, discounts and more. Sign Up. Subscribe to Our Blog. Learn about the latest plasmid technologies and research tools. Subscribe. Contact Addgene. Have questions about your order, deposit, or a plasmid?
Psicheck-2 promega
Did you know?
WebJan 1, 2014 · To generate luciferase-based reporter plasmids, psiCHECK-2 (Promega) was modified by inserting the Gateway cassette C.1 (Invitrogen) at the 3′-end of the firefly luciferase gene (f-luc) into the Xba I site of psiCHECK-2. 2. The 3′-UTR sequence of Tlr2 was amplified from HEK293 cell genomic DNA. 3. Add the attB1 and 2 sequences. 4. WebAug 14, 2015 · zt211 psiCHECK-2 载体 Promega RNAi 系统载体. zt212 pmir GLO 载体 Promega RNAi 系统载体. zt213 pSuper-puro 载体 Oligoengine RNAi 系统载体. zt214 pGenesil-1 载体 RNAi 系统载体. zt215 pmR mCherry 载体 Clontech RNAi 系统载体. zt216 pmiRZip anti-microRNA 载体 SBI RNAi 系统载体
Web主要试剂：BCA蛋白浓度检测试剂盒、RIPA(强)蛋白裂解液、山羊抗兔二抗(中国碧云天生物技术研究所)，性别决定区Y框蛋白5(sex determining region Y-box protein 5, SOX5)抗体、兔抗人甘油醛-3-磷酸脱氢酶(glyceraldehyde-3-phosphate dehydrogenase, GAPDH)抗体(美国Abcam公司)，psiCHECK-2荧光 ... WebYou've created a Promega.com account. Check your inbox to complete email verification. Note: You will not be able to access your account until your email is verified. Close Existing Account Found. ... psiCHECK™-2 Vector C8021 PDF (190 KB) – English (United ...
WebFeb 23, 2024 · 2.1各组大鼠动脉损伤组织vsmcs分离鉴定 正常组织和动脉损伤组织vsmcs中均可见α-sma阳性信号，表明所分离细胞为vmscs。见图1。 2.2mir-448和sirt1在动脉损伤组织vsmcs中的表达 与对照组相比，动脉损伤组织vsmcs中mir-448表达明显上调(p0.05)，sirt1表达明显下调(p0.05)。见图2。 WebThe psiCHECK™-2 Vector is designed to provide quantitative and rapid optimization of RNA interference (RNAi). The vector enables monitoring of changes in expression of a target gene fused to a reporter gene. Renilla luciferase is used as the primary reporter gene, and the gene of interest is cloned into a multiple cloning region located ...
WebThis firefly reporter cassette has been specifically designed to be an intraplasmid transfection normalization reporter; thus when using the psiCHECK™-2 Vector, the Renilla luciferase signal can...
god so loved the world lloyd larsonWebAug 13, 2024 · The 3′UTR of CREBL2 and PCBP2 were sub-cloned into the psiCHECK-2 vector (Promega, USA) downstream of the Renilla luciferase reporter gene. Flanking sequence on each side was synthetized with a short extension containing cleavage sites for XhoI (CTCGAG) and NotI (GCGGCCGC), and the primer sequences are shown in Table … god songs free download tamilWebPsicheck 2 Luciferase Reporter Vector, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … booklive appWebpsiCHECK™-2 Vector. Luciferase reporter vector for monitoring RNA interference effects. C8021. GloSensor™ Technology. Live-cell biosensors for cAMP, cGMP or protease activity. E1290, E1291, E2301, E1171 god song lyrics hillsong unitedWebpsiCHECK-2 (Search Vector Database) Backbone manufacturer Promega Backbone size w/o insert (bp) 6273 Total vector size (bp) 6302 Vector type Mammalian Expression, Luciferase ; microRNA activity Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number High Copy Gene/Insert booklive.jp downloaderWebThe psiCHECK™-1 Vector and psiCHECK™-2 Vector provide a quantitative and rapid approach to optimize RNA interference (RNAi). The vectors enable monitoring of changes in expression of a target gene fused to the reporter gene. book little princessWebNov 3, 2024 · psiCHECK™-2 Vector（C8021）最初是为验证 siRNA 靶标设计的。 该载体用海肾萤光素酶（hRluc）作为主报告基因；萤火虫萤光素酶（hluc+）则作为内参 报告基因，用于进行归一化处理。 目的基因被克隆至海肾萤光素酶翻译终止密码子下游的多克隆位点。 由合成的 siRNA 或体内表达的 shRNA 引发的针对目的基因的 RNAi 过程，导致融合 … booklive line