Percp emission
Web♦ CY 7 has a fluorescence emission maximum around 767nm. PerCP-CY 5.5 490nm max. absorption 695nm max. emission Argon Ion LASER (488nm) ♦ PerCP-CY 5.5 is a tandem conjugate system, combining PerCP and a cyanine dye. ♦ Excitation of CY 5.5 is about 675 (same as PerCP emission) and has a fluorescence emission maximum around 695nm Web* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. Excitation Laser Blue Laser (488 nm) Application Notes ... BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our …
Percp emission
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WebPeridinin Chlorophyll (PerCP) 477 678 488 ... Nucleic Acid Dyes Max. excitation Max. emission Excitation laser wavelength (nm) wavelength (nm) lines (nm) DAPI 359 461 325,360,405,407 Hoechst 33258 352 461 Hoechst 33342 350 461 SYTOX Blue 431 480 YOYO-1 491 509 SYTOX Green 504 523 WebFor example, two of the most common fluorochromes, FITC (blue) and PE (orange) have significant overlap. With conventional flow cytometers the overlapping signal is subtracted decreasing the overall detection efficiency, With antigens expressed at a high density such as CD4 this is not a problem.
WebPeridinin-Chlorophyll-Protein (PerCP) is a red-emitting fluorescent protein isolated from algae that can be excited by the 488 nm blue laser and captured with a 670/30 nm bandpass filter. PerCP exhibits a large stoke's shift, with an excitation peak at 482 nm and an emission peak at 675 nm.
Webspecific compensation controls. Due to its emission spectra, PE-CF594 has significantly less spillover into the PerCP and PE-Cy™7 detectors than PE-Alexa Fluor® 610 (Table 1). These improved spectral characteristics make PE-CF594 an optimal choice for multicolor panels. Compatible with standard surface and intracellular staining protocols Webin less spillover into the violet channels compared to PerCP-Cy5.5. Due to similar excitation and emission properties, BD Horizon BB700 and PerCP-Cy5.5 cannot be used simultaneously. • • • • • • • • • • 488 nm 532 nm 695/40 PerCP-Cy™5.5 (Ex max 482 nm/Em max 678 nm) is a tandem conjugate that combines PerCP with the ...
WebExcitable by the blue 488 nm laser and emitting at 700 nm, it can be detected using the 692/80 filter on the ZE5 Cell Analyzer or similar filters in other instruments. SBB700 is not a tandem dye and exhibits reduced excitation from the 640 nm laser compared to fluorophores with similar excitation and emission (Figure 1). Fig. 1.
Web* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. Application Notes Additional reported application (for relevant formats) include: spatial biology (IBEX) 1,2. Additional Product Notes BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. court dockets grand bankWebExcitation and emission spectra of Alexa Fluor ® 488, PE, PerCP-Cy5.5, and PE-Cy7 following excitation with the blue laser (488 nm). Here are some hints and tips on how to handle tandem dyes: Always protect tandem dyes from … court docket meaningWebPerCP (Peridinin chlorophyll) is a fluorescent compound with an excitation peak at 477 nm and an emission peak at 678 nm, giving it a fairly large Stokes' Shift of 201nm. It can be … brian kelly solicitor belfastWebBasically, light emitted from the first fluorochrome (PE) excites the second (Texas Red), which then fluoresces at a 615 nm maxima. Special care must be taken when using PE … court dockets graves county kyhttp://www.rhlccflow.facilities.northwestern.edu/files/2011/09/Fluorochrome.pdf brian kelly southern drawlWebstaining than other tandem dyes, PerCP-Cy5.5 can be used when Fc receptors are present in the negative cell population. Note: Depending upon instrument settings, some PerCP signal may be detected in the APC channel, requiring cross-laser compensation. PerCP … brian kelly southern accentWebEmission maximum: 676 nm; Extinction coefficient (ε): 1.96 x 10 6 M −1 cm −1; A 483 /A 280 ≥ 4.6; Applications. Peridinin chlorophyll (PerCP) is commonly used in … brian kelly salary 2022