Mcherry-gfp-lc3b质粒

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August undefined, 2024

Web使用 Premo 自噬检测试剂和传感器（基于 LC3B 或 p62 连接荧光蛋白嵌合体或 RFP-GFP-LC3B 串联传感器），可轻松监测细胞自噬的不同阶段。使用 Premo 自噬检测试剂盒，您可选择 Premo 自噬传感器，其基于自噬相关蛋白 LC3B 或 p62、绿色 (GFP) 和/或红色荧光蛋白 (RFP) 嵌合体且具有 BacMam 技术的转导效率。 WebFUW mCherry-GFP-LC3 : 出品公司: 爱迪基因: 目录编号: 110060: 存储实验室: Anne Brunet: 载体骨架基本信息: 载体名称: FUW: 载体出品公司:--载体抗性: 氨苄青霉素: 空载体大小:--完整质粒大小:--载体修饰:--载体类型: 慢病毒载体: 筛选标记:--高拷贝/低拷贝:--生长条件: 37 ...

pCMV-mCherry-GFP-LC3B(pCMV-mCherry-GFP-LC3B质粒)(D281…

Web14 aug. 2015 · 自噬双标腺病毒（mRFP-GFP-LC3）使用指南汉恒生物科技(上海)有限公司400-092-0065地址：上海市徐汇区斜土路1175号景泰大厦1503021-54121689实验室：上海市张江高科技园区张江药谷孵化器1号楼314汉恒生物科技邮箱：[email protected]自噬双标腺病毒（mRFP-GFP-LC3）使用指南背 … WebpCMV-mCherry-GFP-LC3B是碧云天研发的在哺乳动物细胞中表达红色mCherry和绿色GFP双标签的人源LC3B融合蛋白的质粒。该质粒含有CMV启动子，为卡那霉素抗性，转染后能够在靶细胞中高效表达带有红色荧光蛋白mCherry和绿色荧光蛋白GFP双重标签的LC3B的融合蛋白，呈现明亮的 las vegas health card appointments

维真 mCherry-GFP-LC3B 自噬双标腺相关病毒产品说明 - Bio-Equip

Web19 feb. 2024 · Tandem constructs have been designed that couple pH-stable fluorophores like RFP or mCherry with the pH sensitive fluorophore, GFP, to monitor LC3 turn over in the lysosome. Attempts to quantify LC3 foci with microscopy can introduce experimenter bias, so these flux assays are qualitative at best. Web28 mrt. 2024 · 为什么大家都用gfp-lc3去转染细胞，可是这样应该是外源性的表达啊，真的能反应细胞本身的自噬状态吗？为什么没有人用免疫荧光的方法检测lc3呢，而且免疫荧光的方法自认为好像比转染要容易些吧，特别是lamp与lc3共定位的时候，免疫荧光是必然能用到的实验方法，所以为什么一定要转染呢？ http://www.wzbio.com.cn/special.php?cid=44 henrico high school basketball schedule

顺铂通过增加HeLa人宫颈癌细胞中Nrf2入核促进细胞自噬和凋亡

WebTandem GFP-mCherry-LC3B expression indicates elevated autophagic flux in EGFRvIII + (K) BS153 and (L) DKMG cells during hypoxia (24 h O 2 0.02%). Scale bars: 2 μm. Autophagy inhibition through CQ addition (5 µg/ml) abrogates the survival advantage of EGFRvIII + (M) BS153 and (N) DKMG cells as assessed by crystal violet staining after … WebPlasmid pBABE-puro mCherry-EGFP-LC3B from Dr. Jayanta Debnath's lab contains the insert microtubule-associated protein 1 light chain 3 beta and is published in EMBO Rep. 2009 Feb;10(2):173-9. doi: 10.1038/embor.2008.238. Epub 2009 Jan 16. This plasmid is available through Addgene. las vegas helicopter machine gun tourWeb1 mrt. 2024 · mCherry (红光) -eGFP (绿光) -LC3B 串联荧光蛋白可用于检测自噬流水平的融合蛋白。它利用了酸性自溶酶体和中性自噬体之间的 pH 差异以及不同荧光素对 pH 的敏感性差异，以监控从自噬体到自溶酶体的进程 (图 6a) 。图 6. 串联荧光探针及荧光共定位检测自噬流【a: mCherry-eGFP-LC3B 串联荧光探针示意图与机制 [6] ；b: p27 +/+ 和p27 -/- … henrico high school graduation 2019

"Web2 jun. 2011 · A tandem fusion of mCherry and GFP is fused to LC3B (one of the several members of the mammalian LC3 family) to make a pH-sensitive sensor that is used to monitor autophagy in live cells. The GFP tag is acid-sensitive while the … " - Mcherry-gfp-lc3b质粒

Mcherry-gfp-lc3b质粒

WebJournal: Cell Death & Disease Article Title: NDRG1 inhibition sensitizes osteosarcoma cells to combretastatin A-4 through targeting autophagy doi: 10.1038/cddis.2024.438 Figure Lengend Snippet: CA-4 induces autophagy in OS cells. ( a ) SJSA and MG63.2 cells were treated with various concentrations of CA-4, and the whole-cell lysates were subjected to … http://www.biofeng.com/adjy/zhili/pBABE-puro%20mCherry-EGFP-LC3B.html

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http://img1.bioon.com/news/showarticle.asp?newsid=98676 Web12 mei 2024 · mCherry-GFP-LC3B自噬双标系统的工作原理为：未发生自噬的细胞及含有自噬体的细胞中，由于mCherry与GFP共同表达，细胞呈现黄色荧光。当自噬体与溶酶体融合形成自噬溶酶体后，酸性的溶酶体环境使酸敏感的GFP荧光淬灭，而mCherry不受影响，进而使自噬溶酶体呈现红色荧光。因此，红色荧光可指示自噬溶酶体形成的顺利程度 …

WebFUW mCherry-GFP-LC3. Plasmid. #110060. Purpose. To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid … Web细胞自噬形成时，GFP-LC3或mCherry-GFP-LC3融合蛋白转移至细胞自噬体膜，在荧光显微镜下形成多个明亮的绿色或黄色荧光斑点。当细胞自噬溶酶体形成后，酸性的溶酶体环境使GFP荧光淬灭，而mCherry荧光不受影响，细胞自噬溶酶体呈现红色荧光（图6）。

WebGFP-LC3单荧光和mCherry-GFP-LC3双荧光指示系统. 自噬形成时，GFP-LC3或mCherry-GFP-LC3融合蛋白转移至自噬体膜，在荧光显微镜下形成多个明亮的绿色或黄色荧光斑点。当自噬溶酶体形成后，酸性环境使GFP荧光淬灭，而mCherry荧光不受影响，自噬溶酶体呈现红色荧光（图7）。 WebpCMV-mCherry-GFP-LC3B(pCMV-mCherry-GFP-LC3B质粒)(D2816-100μg) pCMV-mCherry-GFP-LC3B是碧云天研发的在哺乳动物细胞中表达红色mCherry和绿色GFP双标签的人源LC3B融合蛋白的质粒。. 该质粒含有CMV启动子，为卡那霉素抗性，转染后能够在靶细胞中高效表达带有红色荧光蛋白mCherry和绿色 ...

WebQ. mCherry-EGFP-LC3 plasmid관련 제가 아직 autophagy를 잘 몰라서 질문드립니다. 이 논문에서 mCherry-EGFP-LC3 retrovirus를 만들어서 infection 시켜 autolysosome의 존재를 확인하는데, Figure1에서 cytoplasm전체적으로 GFP가 발현하는 것을 볼 수 ...: A. 여기에서 virus는 원하는 유전자의 delivery 수단으로 이용된 것 뿐입니다.

WebmCherry-GFP-LC3B腺相关病毒四．维真自噬双标系统的工作原理未发生自噬的细胞及含有自噬体的细胞中，由于mCherry与GFP共同表达，细胞呈现黄色荧光。当自噬体与溶酶体融合形成自噬溶酶体后，酸性的溶酶体环境使酸敏感的GFP荧光淬灭，而mCherry不受影响，进而使自噬溶酶体呈现红色荧光。因此，红色荧光可指示自噬溶酶体形成的顺利程度 … henrico high school girls basketballWebpCMV-GFP-LC3B(pCMV-GFP-LC3B质粒)(D2815-1μg) pCMV-GFP-LC3B是碧云天自行研发的在哺乳动物细胞中表达GFP标签的人源LC3B 融合蛋白的质粒。. 该质粒含有CMV启动子，为卡那霉素抗性，转染细胞后能够在靶细胞中高效表达绿色荧光蛋白GFP标签的LC3B的融合蛋白，呈现明亮的绿色 ... henrico high school graduationWebGreen fluorescent protein (GFP)-LC3B adenovirus, GFP-red fluorescent protein (RFP)-LC3B adenovirus and mCherry-EGFP-LC3 adeno-associated virus were obtained from Hanbio (Shanghai, China). Cell Culture. The human HSC cell line LX-2 (Procell, Wuhan, China) was cultured in DMEM supplemented with 10% FBS at 37 °C and 5% CO 2. henrico high school ibWeb8 jul. 2024 · Inmortalized Human Chondrocytes (T/C28a2) were employed to develop an autophagy reporter cell line using LC3 as a fluorescent reporter (mCherry-EGFP-LC3B) for autophagy. Figure 1 shows the schematic workflow of the screening assay starting with the development of the autophagy reporter cell line in human chondrocytes (mCherry-EGFP … las vegas home and garden show 2022WebFUW mCherry-GFP-LC3 was a gift from Anne Brunet (Addgene plasmid # 110060 ; http://n2t.net/addgene:110060 ; RRID:Addgene_110060) For your References section: Lysosome activation clears aggregates and enhances quiescent neural stem … henrico high school hoursWeb11 mei 2024 · mCherry (红光)-eGFP (绿光)-LC3B 串联荧光蛋白可用于检测自噬流水平的融合蛋白。它利用了酸性自溶酶体和中性自噬体之间的 pH 差异以及不同荧光素对 pH 的敏感性差异，以监控从自噬体到自溶酶体的进程 (图 6a)。图 6. 串联荧光探针及荧光共定位检测自噬流【a: mCherry-eGFP-LC3B 串联荧光探针示意图与机制 [6]；b: p27+/+ 和p27-/- … henrico high school in a videoWeb26 jun. 2024 · VigenemCherry-GFP-LC3B自噬双标慢病毒产品说明一．关于自噬及LC3 1. 自噬大自噬（macroautophagy），也就是通常说的自噬（autophagy），是真核细胞蛋白降解的途径之一。自噬可以被描述为细胞质内的成分（细胞器、蛋白等）被双层膜的囊泡包裹，形成自噬体（autophagosome），进而传递到溶酶体进行降解的过程。 las vegas headliner shows 2023